PRSS12 is a member of the trypsin family of serine proteases.
Studies in mouse suggest that the encoded enzyme may be involved in structural reorganizations associated with learning and memory.
The enzyme is also expressed in Leydig cells in the testis.
Proba et al.
(1998) cloned PRSS12 from a human fetal brain cDNA library, using primers based on a conserved region of the mouse homolog cloned by Gschwend et al.
(1997).
The PRSS12 cDNA encodes a deduced 875aa protein that shares 82.5% overall sequence identity with the mouse protein.
Human PRSS12 contains a putative signal peptide, a kringle domain, 4 repeated scavenger receptor cysteine-rich (SRCR) regions, and a C-terminal serine protease domain.
Comparison of PRSS12 with other proteases identified it as a trypsin-like serine protease.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PRSS12.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu17-Ser235 of human PRSS12, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
