PKX1 appeared to encode a novel type of protein kinase that is related to the catalytic subunit of cAMP-dependent protein kinases and has striking homology to the DC2 protein kinase of Drosophila melanogaster.
PKX1 is part of a family of at least 4 genes or pseudogenes, of which 3 map to the human sex chromosomes.
In contrast to all other genes from the X-specific region of Xp22.3, PKX1 has a homolog on Yp (PRKY) rather than Yq.
This suggests that the single pericentric inversion event hypothesized to have occurred during primate evolution is not sufficient to explain the present X/Y homology pattern of Xp22.3.
This suggests that the PKX1 gene, besides harboring a previously described hotspot for illegitimate Xp/Yp recombination, contains additional sequences which predispose the X chromosome to breakage events.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat PRKX.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Trp49-Phe358 of rat PRKX, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
