5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme belongs to the ser/thr protein kinase family.
It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK).
AMPK is a cellular energy sensor conserved in all eukaryotic cells.
The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio.
AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation.
It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PRKAa1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly198-Lys448 of human PRKAa1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
