Previously identified proline-rich proteins have some similarities to collagen and some to enamel protein.
Identification of polymorphism stimulated search for an association between specific phenotype and oral disease.
The genes are rich in HaeIII restriction sites; hence, the designation PRH1 and PRH2.
Maeda (1985) concluded that what she terms PRH2 codes for Pr.
HindIII restriction fragments of DNA from Chinese hamster x mouse somatic cell hybrids hybridized with cDNA clones of proline-rich protein only when the DNA was isolated from cells containing mouse chromosome 8 or a fragment thereof.
PRP is near the centromere.
In the mouse, polymorphism of the proline-rich proteins of saliva is demonstrated to have a relationship to taste.
There was complete concordance between this marker and an RFLP in the 12q14-12q22 region.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PRH2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp17-Gln166 of human PRH2, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
