Perforin is a cytolytic protein found in the granules of CD8 T-cells and NK cells.
Perforin is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells.
Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore.
The lytic membrane-inserting part of perforin is the MACPF domain.
This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria.
Perforin has structural and functional similarities to complement component 9 (C9).
Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells.
This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat PRF1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ala120-Asp353 of rat PRF1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
