Peroxiredoxin-6 is a member of the thiol-specific antioxidant protein family.
This protein is a bifunctional enzyme with two distinct active sites.
It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides.
It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Kim et al.
(1997) isolated a calcium-independent lysosomal PLA2 from a human myeloblast cell line.
The predicted 224aa protein contained the PLA2 catalytic motif, but no other significant homology to known phospholipases.
Expressed protein had significant PLA2 activity on several substrates.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat PRDX6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Pro224 of rat PRDX6, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
