The PR domain is a protein-protein interaction module of about 100aa.
PR domain-containing proteins, such as PRDM14, are often involved in transcriptional regulation.
PRDM14 gene encodes a deduced 571aa protein that is 68% identical to the mouse ortholog
Introduction of PRDM14 into cancer cells with normally low endogenous PRDM14 enhanced cell growth and reduced their sensitivity to various chemotherapeutic drugs.
In contrast, siRNA knockdown of PRMD14 induced apoptosis in breast cancer cells and increased their chemotherapeutic drug sensitivity.
Little, if any, expression of PRMD14 was detected in normal tissues.
PRMD14 may be an ideal therapeutic target for the treatment of breast cancer.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PRDM14.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu395-His568 of human PRDM14, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
