Calcineurin, a calmodulin-regulated protein phosphatase, is found in the cells of all eukaryotes ranging from yeast to mammals.
Wang et al.
(1996) described this heterodimeric protein as having a 19kD Ca(2+)-binding regulatory subunit, calcineurin B, and a 58- to 59kD catalytic subunit, calcineurin A.
One gene encodes calcineurin B in all tissues except testis, and it is highly conserved at the level of both protein and DNA sequences in eukaryotes.
In contrast, there are 2 major isoforms, alpha and beta, of calcineurin A encoded by separate genes located on different human chromosomes.
A third isoform, A-gamma, is unique to testis.
Additional diversity of calcineurin A is created by alternative splicing of mRNAs.
Calcineurin is especially abundant in brain where it constitutes 1% of total protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PPP3R1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly2-Val170 of human PPP3R1, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
