PPOX is a human gene that produces an enzyme called protoporphyrinogen oxidase.
This enzyme is responsible for the seventh step in heme production.
Heme is the portion of hemoglobin that carries oxygen in the blood from the lungs to the rest of the body.
Each of the steps in heme production is controlled by a separate gene.
Protoporphyrinogen oxidase removes hydrogen atoms from protoporphyrinogen IX (the product of the sixth step in the production of heme) to form protoporphyrin IX.
One additional enzyme must modify protoporphyrin IX before it becomes heme.
This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX.
This protein is a flavoprotein associated with the outer surface of the inner mitochondrial membrane.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat PPOX.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Gly9-Ala300 of rat PPOX, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
