Phospholipase A2 catalyzes the release of fatty acids from glycero-3-phosphocholines.
The Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported.
One striking feature of these enzymes is their close homology to venom phospholipases of snakes.
Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets.
In analogous human cDNA obtained from human lung, they also obtained and analyzed the apparently unique human gene encoding pancreatic PLA2.
Hybridization probes made from human 'pancreatic' cDNA hybridized to two distinct genomic fragments of cobra DNA, indicating again a striking homology to one or more counterpart in one variety of snake.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human pPLA2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp16-Ser148 of human pPLA2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
