Periplakin is a component of desmosomes and of the epidermal cornified envelope in keratinocytes.
The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments.
This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments.
AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1- mediated signaling.
The deduced 1666aa protein has a calculated molecular mass of about 205kD.
PPL shares extensive sequence homology with other proteins of the plakin family, with highest identities found in the N- and C-terminal domains.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse PPL.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Glu1501-Lys1754 of mouse PPL, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
