Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids.
Galactoside-binding soluble lectin 13 has lysophospholipase activity.
It is composed of two identical subunits which are held together by disulfide bonds.
This protein has structural similarity to several members of the beta-galactoside-binding S-type lectin family.
By ultracentrifugation and SDS-PAGE, LGALS13 showed an apparent molecular mass of 29-30kD.
Under reducing conditions, the apparent molecular mass was 16kD, suggesting that LGALS13 is composed of 2 identical subunits held together by disulfide bonds.
Western blot analysis and Ouchterlony gel diffusion were unable to detect LGALS13 in serum or in any other adult or fetal tissue examined.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PP13.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Asn139 of human PP13, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
