84-4433-31 POTEG (Ankyrin Domain Family, Member G, A26C2, POTE14, POTE14a, CT104.4, ANKRD26-Like Family C Member 2, Cancer/Testis Antigen Family 104,Member 4, Prostate, ovary, testis-expressed protein on chromosome 14) 200ul 364719
84-4433-31 POTEG (Ankyrin Domain Family, Member G, A26C2, POTE14, POTE14a, CT104.4, ANKRD26-Like Family C Member 2, Cancer/Testis Antigen Family 104,Member 4, Prostate, ovary, testis-expressed protein on chromosome 14) 200ul 364719
The deduced 508aa POTE14A protein has a calculated molecular mass of 57.2kD.
POTE14A has 3 cysteine-rich N-terminal repeats, 6 central ankyrin repeats, and a C-terminal helical region.
The deduced 594aa POTE15B protein has a calculated molecular mass of 67.1kD.
Compared with POTE14A, POTE14B has extra sequence within the ankyrin repeat region.
The deduced 487aa POTE14C protein has a calculated molecular mass of 54.6kD.
Compared with POTE14A, POTE14C has a C-terminal truncation.
In vitro transcription and translation of the POTE14 variants resulted in proteins with molecular masses in agreement with their calculated masses.
By partially sequencing clones obtained from prostate and testis cDNA libraries, they determined that the POTE14 gene is transcribed.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes human POTEG.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to Leu177-Asp330 of human POTEG, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.