By screening human placenta and osteosarcoma cDNA libraries with mouse Postn as probe, they cloned 2 variants of human POSTN.
One variant encodes a deduced 779aa protein with an apparent molecular mass of 87.0kD, and the other encodes a deduced 836aa protein with an apparent molecular mass of 93.3kD.
POSTN contains a typical signal sequence, followed by a cysteine-rich domain, a 4-fold repeat structure of about 150aa, and a C-terminal domain.
Mouse and human POSTN share 89.2% amino acid identity overall and 90.1% identity in their mature forms.
Northern blot analysis of a mouse osteoblastic cell line detected a 3.4-kb Postn transcript.
RNA dot blot analysis detected expression of Postn in primary mouse osteoblasts and in lung, but not in any other tissues examined.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat POSTN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Arg659-Gln810 of rat POSTN, fused to N-terminal His-Tag expressed in E. coli.
