Members of the paraoxonase (EC 3.1.1.2) gene family, such as PON3, encode high density lipoprotein (HDL)-related glycoproteins with multienzymatic properties.
(Lu et al., 2006).
Lu et al.
(2006) cloned PON3 from a fetal liver cDNA library.
The deduced mature protein has 354aa and a calculated molecular mass of 39.6kD.
It contains 3 cysteines that are conserved in PON1 and PON2.
Cys41 and cys351 are predicted to form an intramolecular disulfide bond, and cys283 is predicted to be involved in antioxidant activity.
Using nondenaturing PAGE, Draganov et al.
(2005) observed native recombinant PON3 at an apparent molecular mass of 94.9kD, suggesting that it probably forms dimers.
Glycosidase treatment of human serum PON3 suggested that the secreted form of PON3 contains complex carbohydrates.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PON3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly2-Leu354 of human PON3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
