(1985) complemented a temperature-sensitive defect in murine POLA by fusing the defective mouse cells with human cells.
A gene present on Xpter-q22 was responsible for correcting the defect.
Wang et al.
(1985) used a monoclonal antibody that distinguishes human from rodent DNA polymerase alpha to study human-rodent cell hybrids.
They mapped the gene to a site near the junction of Xp21.3 and Xp22.1 (perhaps Xp21.12-p21.11) and showed that it is not expressed in an inactive X chromosome.
They concluded that POLA is distal to DMD and CGD but proximal to STS.
They predicted that mosaic nullisomy in a female resulting from deletion of this gene could not exist and that hemizygosity for deletion of the gene in a male would be lethal because of the essentiality of the gene to the catalytic activity of DNA polymerase alpha.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse POLa1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ala1209-Ser1465 of mouse POLa1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
