Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts.
The rate-limiting enzyme in vitamin B6 synthesis is pyridoxine-5-prime-phosphate (PNP) oxidase (PNPO; EC 1.4.3.5)
Pyridoxine 5’-phosphate oxidase is an enzyme that catalyzes several reactions in the vitamin B6 metabolism pathway.
Pyridoxine-5-P oxidase catalyzes the terminal step (also the rate-limiting step) in vitamin B6 metabolism, the biosynthesis of pyridoxal-5’-phosphate, the biologically active form of vitamin B6 which acts as an essential cofactor.
Pyridoxine 5’-phosphate oxidase is a member of the enzyme class oxidases, or more specifically, oxidoreductases.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse PNPO.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Pro261 of mouse PNPO, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
