Nociceptin (orphanin FQ), a neuropeptide of 17aa, is the natural agonist of opioid receptor-like-1 (OPRL1), a G protein-coupled receptor whose human and murine cDNAs had previously been identified.
This neuropeptide is endowed with pronociceptive activity in vivo.
Nociceptin is derived from a larger precursor, prepronociceptin (PNOC) is highly conserved in the 3 species and displays organizational features that are strikingly similar to those for the genes for preproenkephalin, preprodynorphin, and preproopiomelanocortin.
Mollereau et al.
showed that the PNOC gene encodes a single copy of nociceptin as well as another peptide whose sequence is strictly conserved across murine and human species; hence, it is likely to be neurophysiologically significant.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse PNOC.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ser12-Val187 of mouse PNOC, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
