May play a role in sperm morphology especially the sperm tail and consequently affect fertility.
May also be involved in the general organization of cellular cytoskeleton
Cotransfection of PMF1 and NRF2 activated transcription from the polyamine-responsive element of the SSAT promoter in a reporter assay, and PMF1 was the rate-limiting component.
Mutations that interrupted either of these regions altered the ability of the proteins to interact, and they lost their ability to regulate transcription of the SSAT gene.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PMFBP1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp9-Leu206 of human PMFBP1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
