The cDNA sequence (FB7) was isolated by serendipity from a human fetal brain cDNA library.
The SEP gene was expressed at highest levels in fetal kidney
PLXNB1 is a receptor for the transmembrane semaphorin SEMA4D, also called CD100.
Binding of SEMA4D to PLXNB1 stimulated tyrosine kinase activity of MET, resulting in tyrosine phosphorylation of both receptors.
This effect was not found in cells lacking MET expression.
This activity required the interaction of plexin B1 with RND1, a small GTP-binding protein of the Rho family.
Downregulation of RRAS activity by the plexin B1/RND1 complex was essential for the SEMA4D-induced growth cone collapse in hippocampal neurons.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse PLXNB1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Leu1598-Val1849 of mouse PLXNB1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
