Scramblases are members of the general family of transmembrane lipid transporters known as flippases.
The inner-leaflet facing the inside of the cell contains negatively-charged amino- phospholipids and phosphatidylethanolamine.
The outer-leaflet, facing the outside environment, contains phosphatidylcholine and sphingomyelin.
Scramblase is an enzyme, present in the cell membrane, that can transport (scramble) the negatively-charged phospholipids from the inner- leaflet to the outer-leaflet, and vice versa.
Wiedmer et al.
(2000) determined that the PLSCR1 gene contains 9 exons and spans about 30 kb.
The first exon is untranslated.
By deletion analysis of PLSCR1 promoter-reporter constructs, they found that 97% of the promoter activity resides between -95 bp in the 5-prime flanking region to +60 bp of the first exon.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PLSCR1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Trp318 of human PLSCR1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
