The LH3 cDNA clones encode a 738aa polypeptide, including a signal peptide of 24 residues.
The overall aa sequence identity between the processed human PLOD3 and PLOD1 polypeptides is 59%, and that between the processed PLOD3 and PLOD2 polypeptides is 57%, whereas the identity to the processed Caenorhabditis elegans polypeptide is only 45%.
All 4 critical residues at the catalytic site, 2 histidines, 1 aspartate, and 1 arginine, are conserved in all of these polypeptides.
The mRNA for PLOD3 was found to be expressed in a variety of tissues, but distinct differences appeared to exist in the expression pattern of the 3 isoenzyme mRNAs.
Recombinant PLOD3 expressed in insect cells by means of a baculovirus vector was found to be more soluble than PLOD1 expressed in the same cell type.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PLOD3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg647-Pro738 of human PLOD3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
