84-4432-95　PLEKHA1 (Pleckstrin Homology Domain Containing Family A, Member 1, TAPP1, Tandem PH Domain Containing Protein-1, PH domain-containing family A member 1) 200ul　364684

特徴

• The last 3 C-terminal aa of TAPP1 are predicted to interact with PDZ domain-containing proteins.
• TAPP1 and TAPP2 share 58% identity over the first 300aa, which encompass both PH domains.
• There is little homology in the C termini.
• Northern blot analysis detected a 4.0-kb transcript expressed in all tissues examined, with highest levels in skeletal muscle, spleen, lung, thymus, and placenta
• TAPP1 possessed a 5-fold higher affinity for PtdIns(3,4)P2 than TAPP2.
• Mutation of the conserved arg212 to leucine within the PPBM of the second PH domain of TAPP1 reduced or eliminated PtdIns(3,4)P2 binding.
• The N-terminal PH domain contributed to the affinity with which the C-terminal PH domain bound to PtdIns(3,4)P2.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.