By Western blot analysis using an antibody against guinea pig phospholipase B (PLB), Maury et al.
(2002) detected a 97kD polypeptide in the soluble and membrane fractions of human epidermis that was enriched in the soluble fraction.
Consistent with these results, they found that PLB activity was greatest in the soluble fraction.
Trypsin had no effect on the molecular mass or enzymatic activity, suggesting that the protein they detected was already in an active form.
Immunolocalization experiments revealed that PLB is expressed in the entire human epidermis with an accumulation in the dermoepidermis junction.
In situ hybridization also indicated expression in the entire epidermis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PLB.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1036-Leu1458 of human PLB, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
