Sequence analysis revealed that the deduced 1465aa PLA2R1 transmembrane protein contains a 25aa signal sequence, 15 potential N-glycosylation sites, an N-terminal cysteine-rich domain, a fibronectin-like type II domain, 8 carbohydrate recognition domains, and a short C-terminal cytoplasmic tail containing a consensus sequence (asn-pro-X-tyr) also found in bovine Pla2r1.
A casein-kinase II phosphorylation site is also located in this C-terminal region.
Ancian et al.
(1995) identified a splice variant encoding a soluble form of PLA2R1.
Quantitative PCR analysis suggested that the ratio for the transcription of membrane to soluble PLA2R1 is 1.6.
Northern blot analysis detected strong expression of 6.5- and 5.4-kb PLA2R1 transcripts in kidney, with moderate expression in placenta, lung, and skeletal muscle.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse PLA2R1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Trp1418-Pro1487 of mouse PLA2R1, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
