PLA2G4D acts as a calcium-dependent phospholipase A2 enzyme with a lineolic acid-specific activity.
Using in situ hybridization and immunohistochemistry of normal and psoriatic skin, as well as skin from patients with inflammatory skin conditions such as atopic dermatitis and mycosis fungoides, the authors determined that PLA2G4D mRNA and protein display a strong specific expression in the upper epidermis in psoriatic skin, and not in lower epidermis and dermis.
A similar distribution of PLA2G4D expression, at decreased levels, was detected in the upper epidermis of patients with other inflammatory skin conditions, while PLA2G4D mRNA and protein were not detected in normal adult skin.
PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PLA2G4D.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro9-Leu120 of human PLA2G4D, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
