The A2 phospholipases (PLA2s; EC 3.1.1.4) catalyze the release of fatty acids from phospholipids and play a role in a wide range of physiologic functions.
Cupillard et al.
(1997) cloned a novel secretory PLA2, termed GXSPLA2, from a human fetal lung cDNA library.
The sequence encodes a 165aa polypeptide, including the 42aa signal peptide.
Northern blot analysis revealed that GXSPLA2 expresses an 0.8-kb mRNA in pancreas, lung, and colon and a 1.5-kb mRNA in spleen, thymus, and peripheral blood leukocytes.
Expression studies showed that the cDNA encodes an active phospholipase which was secreted into the culture medium.
Cupillard et al.
(1997) used in situ hybridization to map the PLA2G10 gene to human chromosome 16p13.1-p12.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PLA2G10.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu32-Asp165 of human PLA2G10, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
