Pyruvate Kinase M2 (M2-PK) is strongly overexpressed in a metabolic state specific for a variety of tumor cells.
Recent reports indicate that Tumor M2-PK is a marker for renal cell carcinoma and.
lung cancer.
It has also been investigated in.
pancreatic cancer and gastric cancer with promising results
M2-PK is encoded by the gene PKM2 which does also encode for the pyruvate kinase isoenzyme type M1 (PKM1 or M1-PK).
The M-gene consists of 12 exons and 11 introns.
PKM1 and PKM2 are different splicing products of the M-gene (exon 9 for PKM1 and exon 10 for PKM2) and solely differ in 23aa within a 56aa stretch (aa378-434) at their carboxy terminus.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PKM2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg294-Phe470 of human PKM2, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
