Protein kinase inhibitor Gamma is a member of the PKA inhibitor family.
Studies of a similar protein in mice suggest that this protein acts as a potent competitive PKA inhibitor, and is a predominant form of PKA inhibitors in various tissues.
Three alternatively spliced transcript variants encoding the same protein have been reported.Most of the amino acid residues in the domain of PKI-gamma that showed affinity for the catalytic subunit are conserved, and PKI-gamma also has a region highly similar to the consensus PKI nuclear export signal.
Northern blot analysis detected a 1.3-kb transcript in all tissues examined, with highest expression in heart, skeletal muscle, and testis.
The authors verified the presence of PKI-gamma protein in mouse tissues by purifying it from heart using DEAE chromatography.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PKIg.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ser76 of human PKIg, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
