cAMP-dependent protein kinase inhibitor beta is a member of the cAMP-dependent protein kinase inhibitor family.
Studies of a similar protein in rat suggest that this protein may interact with the catalytic subunit of cAMP-dependent protein kinase and act as a competitive inhibitor.
At least three alternatively spliced transcript variants encoding the same protein have been reported
The deduced 70aa protein has a calculated molecular mass of about 7.4kD.
The purified protein has an apparent molecular mass of 10.5 by SDS-PAGE.
Using kinetic analysis for inhibitor activity, Van Patten et al.
(1991) determined that purified PKIB is a competitive inhibitor of cAMP-dependent protein kinase.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PKIb.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met8-Glu76 of human PKIb, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
