By screening a placenta cDNA library using a PCR fragment derived from the second conserved domain within the catalytic site of ser/thr protein kinases, Johannes et al.
obtained a full-length cDNA encoding PKC-mu.
The deduced 912aa PRKCM protein has a calculated molecular mass of 102kD.
PRKCM has 2 characteristic cysteine-rich repeats, which are conserved among nearly all members of the PKC family, a conserved ATP-binding consensus sequence in the kinase domain, and the invariate aspartate essential for kinase activity.
Northern blot analysis detected a single PRKCM transcript of 3.8 kb at low, constitutive levels in all tissues examined.
The authors verified PRKCM kinase activity by transfection of a PRKCM expression vector and detecting changes in protein phosphorylation patterns by Western blot analysis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PKD1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Thr651-Gln876 human PKD1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
