The genes encoding the protein kinase C enzymes are widely distributed, e.g., PRKCA on chromosome 17, PRKCB1 on 16, and PRKCG on 19.
Although the sequence homology among the PRKC family of genes is extensive, the pattern of expression varies among tissues.
For example, the delta polypeptide appears to be the major isoform expressed in mouse hematopoietic cells.
Mischak et al.
(1991) isolated and characterized the mouse Prkcd gene.
Aris et al.
(1993) found that PKC-delta underwent calcium-independent autophosphorylation in the presence of phosphatidylserine and diacylglycerol.
Diacylglycerol was an absolute requirement for PKC-delta activation.
This and other cofactor and substrate requirements distinguished human PKC-delta from its mouse homolog.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse PKCd.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Asn328-Ile674 of mouse PKCd, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
