Phosphatidylinositol transfer protein beta isoform is found in the cytoplasm, where it catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes.
Tanaka et al.
(1995) cloned PITPNB cDNA by screening a human brain cDNA library with the rat brain cDNA homolog as probe.
The deduced 271aa protein has a calculated molecular mass of 31.5kD and shows 98.1% sequence identity with the rat protein.
Northern blot analysis detected ubiquitous expression of a 3.4-kb transcript, with highest expression in liver and lowest in skeletal muscle.
Expression was found in all regions of the brain examined, with highest expression in amygdala.
The International Radiation Hybrid Mapping Consortium mapped the PITPNB gene to chromosome 22.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PITPNb.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Val2-Val271 of human PITPNb, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
