The hormonally responsive prolactin-inducible protein gene is expressed in benign and malignant breast tumor tissues and in some normal exocrine organs such as sweat, salivary, and lacrimal glands.
The PIP gene is 7 kb long with 4 exons ranging from 106 bp to 223 bp
PIP is overexpressed in 80% of primary and metastatic breast cancers, is duplicated in the breast carcinoma cell line T47D.
The 2 copies are organized as a large palindrome, lying 'in loco' on one chromosome 7.
The FRA7I fragile site lies 2 Mb telomeric to the PIP gene and sets the distal end of the repeated sequence.
Breakage at the FRA7I site was repaired by 1 cycle of the breakage--fusion-bridge (BFB) cycle mechanism, resulting in amplification of the PIP gene.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat PIP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Asn41-Thr139 of rat PIP, fused to N-terminal His-Tag expressed in E. coli.
