The PINP molecule is similar to PIIINP consisting of three distinct structural domains: Col 1 is on the aminoterminal side of the molecule, while Col 2 and Col 3 are situated on the middle of the helically structured molecule (Kühn et al.
1982).
The PINP molecule has a molecular mass of 35 000 and is cleared by scavenger receptors in liver endothelial cells (Melkko et al.
1994).
PINP often occurs in circulation in two forms of different molecular sizes.
One is identical to the trimeric authentic antigen (intact PINP) whereas the other consists of smaller forms of PINP, resembling a single domain of the proalpha1(I) chain of PINP and is probably a degradation product of type I procollagen or I pN-collagen.
Thus, an assay of intact PINP rather than total PINP appears to be more sensitive in detecting changes in the rate of type I collagen synthesis (Melkko et al.
1996, Risteli & Risteli 1999).
Applications:Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 5-20ug/ml Immunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes mouse PINP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC
Calc Crossreactivity：Mo
Immunogen：Small molecule, PINP, conjugated to OVA.
