84-4432-52　［受注停止］PIK3C3 (Phosphoinositide-3-Kinase Class 3, Vps34, Phosphatidylinositol 3-kinase p100 subunit, Phosphatidylinositol 3-kinase catalytic subunit type 3) 200ul　364641

特徴

• Phosphoinositide (PI) 3-kinases are involved in both receptor-mediated signal transduction and intracellular trafficking.
• In yeast, the vps34 gene product is a PI 3-kinase that mediates the active diversion of proteins from the secretory pathway to vacuoles.
• Mammals appear to have a family of PI 3-kinases, which also includes PIK3CG and PIK3CA.
• The full-length PIK3C3 cDNA encodes an 887aa polypeptide that is 37% identical to yeast vps34 over its entire length.
• Its predicted and observed molecular mass is approximately 100kD.
• PIK3C3 gene was expressed as a 3.7-kb transcript in all human tissues examined.
• The PIK3C3 gene does not associate with the p85 regulatory subunit but does form a complex with p150, a mammalian homolog of another protein in the yeast vps pathway.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- YPVIFKHGDD LRQDQLILQI ISLMDKLLRK ENLDLKLTPY KVLATSTKHG FMQFIQSVPV AEVLDTEGSI QNFFRKYAPS ENGPNGISAE VMDTYVKSCA GYCVITYILG VGDRHLDNLL LTKTGKLFHI DFGYILGRDP KPLPPPMKLN KEMVEGMGGT QSEQYQEFRK QCYTAFLHLR RYSNLILNLF SLMVDANIPD IALEPDKTVK KVQDKFRLDL SDEEAVHYMQ SLIDESVHAL FAAVVEQIHK FAQYW
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.