E3 SUMO-protein ligase PIAS3 is a member of the PIAS family of transcriptional modulators.
The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates.
It directly binds to several transcription factors and either blocks or enhances their activity
Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined.Gel-shift assays showed that PIAS3 can block MITF DNA-binding activity, and cotransfection of MITF and PIAS3 in NIH 3T3 cells inhibited MITF-driven transcription activity.
Interaction between PIAS3 and MITF was independent of STAT3 binding.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PIAS3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Val11-Leu280 of human PIAS3, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
