Phosphoglycolate Phosphatase (EC 3.1.3.18) may have an important regulatory influence on oxygen transport in man by indirectly affecting the level of red cell 2,3-diphosphoglycerate.
(The spellings 'phosphoglycolate' and 'phosphoglycollate' have been used interchangeably, but the former is preferred.) Barker and Hopkinson (1978) devised a method for detecting PGP isozymes after starch-gel electrophoresis.
They are present in all human tissues, with highest activities in skeletal and cardiac muscle.
Six different electrophoretic phenotypes were identified.
Family studies showed that these are determined by 3 alleles at an autosomal locus.
In a sample of Europeans, the frequency of the alleles were PGP(1), 0.826; PGP(2), 0.129; PGP(3), 0.045.
The 3-banded isozyme pattern in heterozygotes suggested that the enzyme is dimeric.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse PGP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Leu81-Gly321of mouse PGP, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
