Phosphoglucomutases (PGM) catalyze the transfer of phosphate between the 1 and 6 positions of glucose.
Isozymes of PGM are monomeric, with molecular masses of about 60kD, and are encoded by several genes, including PGM1.
In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity.
One exception is red cells, where PGM2 is a major isozyme.Whitehouse et al.
isolated a cDNA encoding human PGM1.
Eighteen amino acid differences were found between human and rabbit PGM1.
Southern blot analysis indicated that PGM1 is conserved among a wide variety of vertebrates ranging from primates to birds and amphibia.
No evidence for PGM1-related sequences was found either by Southern blot analysis or by in situ hybridization.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PGM1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Lys235 of human PGM1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
