Innate nonself immune recognition relies on structures common among invading microbes, a process termed pattern recognition.
Peptidoglycan is a fundamental component of the bacterial cell wall and is thus a candidate for a pattern recognized by the immune system
The deduced human PGLYRP protein has 196aa.
Both the human and mouse PGLYRP proteins share 43% sequence identity with the T.
ni PGLYRP protein.
Recombinant mouse Pglyrp protein expressed in insect cells possessed an affinity for peptidoglycan.
Dot blot analysis of a number of human tissue mRNAs detected strong expression in bone marrow and weak expression in lung, kidney, liver, small intestine, spleen, thymus, peripheral leukocyte, and fetal spleen.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- FI VPRSEWRALP SECSSRLGHP VRYVVISHTA GSFCNSPDSC EQQARNVQHY HKNELGWCDV AYNFLIGEDG HVYEGRGWNI KGDHTGPIWN PMSIGITFMG NFMDRVPAKR ALRAALNLLE CGVSRGFLRS NYEVKGHRDV QSTLSPGDQL YQVIQSWEHY RE
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse PGLYRP1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Phe19-Glu182 of mouse PGLTRP1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
