Phosphoglyceric acid mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway.
The fact that the PGAMA and GOT1 loci are linked in the mouse supports the assignment of PGAMA to human chromosome 10.
PGAM is a dimeric enzyme containing, in different tissues, different proportions of a muscle (MM) isozyme, a brain (BB) isozyme, and a hybrid form (MB).
Electrophoresis of normal adult human muscle PGAM shows marked predominance of the MM band with only faint BB and MB bands.
In most other human tissues, including brain, liver, erythrocytes, and leukocytes, PGAM-BB is the only demonstrable isozyme.
In cardiac muscle extracts, all 3 bands are seen, although PGAM-MM predominates.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PGAM1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala2-Lys254 of human PGAM1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
