Members of the profilin family of actin-binding proteins, including PFN3, are key regulators of actin dynamics in living cells.
By database analysis using the rat Pfn3 cDNA as query, Braun et al.
(2002) identified human and mouse PFN3.
The deduced 137aa human protein shares 89% amino acid identity with the mouse protein.
Northern blot analysis and RT-PCR of mouse tissues and testis detected Pfn3 expression in testis only.
In situ hybridization of mouse testis detected Pfn3 expression specifically in spermatocytes in the last stages of spermatogenesis
Braun et al.
(2002) determined that the PFN3 gene contains a single exon.
It is in head-to-head orientation with the SLC34A1 gene, which is on the opposite strand.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PFN3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Arg133 human PFN3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
