Peroxisome assembly factor 1 is a protein encodes an integral peroxisomal membrane protein required for peroxisome biogenesis.
The protein is thought to be involved in peroxisomal matrix protein import.
Mutations in this gene result in one form of Zellweger syndrome and infantile Refsum disease.
Alternative splicing results in multiple transcript variants encoding the same protein.Shimozawa et al.
(1992) demonstrated a point mutation in peroxisome assembly factor-1 (PAF1), a 35kD peroxisomal membrane protein (PMP35) for which the cDNA had been cloned by Tsukamoto et al.
(1991).
They had previously shown that this protein corrected the Zellweger-like defect in peroxisome assembly in a peroxisome-deficient Chinese hamster ovary mutant cell line (Z65).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
AA Sequence:MGHHHHHHSG SEF- ALE QLVWSQFTQC FHGFKPGLLA RFEPEVKACL WVFLWRFTIY SKNATVGQSV LNIKYKNDFS PNLRYQPPSK NQKIWYAVCT IGGRWLEE
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PEX@.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala28-Glu118 of human PEX2, expressed in E. coli.
