PES1 is abnormally elevated in malignant tumors of astrocytic origin.
It is a strongly conserved gene containing a BRCT domain that is essential for the activity of this gene product.
The gene plays a crucial role in cell proliferation and may be necessary for oncogenic transformation and tumor progression.
The deduced 588aa PES1 protein is 74% identical to the zebrafish sequence.
The authors noted the presence of a highly acidic region at the C terminus and that the protein has an alpha-helix structure.
Immunoblot analysis showed that PES1 is expressed as an unmodified 72kD protein and a SUMO1-modified 92kD protein.
In situ hybridization analysis revealed wide and high expression of Pes1 in embryonic mouse brain and spinal cord.
Pes1 expression was upregulated in malignant, late-passage mouse astrocytes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PES1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ser415 of human PES1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
