Preproenkephalin has 267aa, as does proopiomelanocortin.
Both genes contain 2 introns.
In both, all the repeated enkephalin or melanotropin sequences are encoded by a single large exon (exon 3).
Preproenkephalin mRNA encodes 4 copies of met-enkephalin, 2 copies of met-enkephalin extended sequences, and 1 copy of leu-enkephalin.
Each copy is flanked by paired basic amino acids which are presumably recognized by the processing protease
The corresponding amino acid sequence shows that the precursor is 267aa long and contains 6 interspersed Met-enkephalin sequences and 1 Leu-enkephalin sequence.
The precursor does not contain the sequences of dynorphin, alpha-neo-endorphin or beta-endorphin.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PENK.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu25-Phe267 of human PENK, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
