RKIP cDNA encodes a protein of 186aa, the sequence of which is 95% identical to the bovine 21- to 23kD protein.
The rat protein shows 85.5% identity with the human protein.
highly expressed in the brain and associated with cytosolic proteins and small GTP-binding proteins.
Multiple tissue Northern blots revealed the presence of a single mRNA in the different tissues of each species; a single band of 1.8 kb was identified in human, 1.45 kb in mouse, and 1.2 kb in rat tissues.
The mRNA is particularly highly expressed in rat and mouse testis, where the level was 30 times higher than that in the brain.
PBP is expressed strongly in the cell bodies of oligodendrocytes but only weakly elsewhere.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PEBP1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro2-Lys187 of human PEBP1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
