84-4432-11　PDXK (Pyridoxal Kinase, PKH, PNK, C21orf124, C21orf97, PRED79, Pyridoxine Kinase, Vitamin B6 Kinase) 200ul　364600

特徴

• Pyridoxal (pyridoxine, vitamin B6) kinase is also known as PDXK.
• The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism.
• The encoded protein is cytoplasmic and probably acts as a homodimer.
• Alternatively spliced transcript variants have been described, but their biological validity has not been determined
• Hanna et al.
• (1997) cloned the cDNA encoding PDXK, which they referred to as PKH.
• The PDXK gene encodes a 312aa polypeptide, and expression of the cDNA revealed pyridoxal kinase activity.
• Northern blot analysis revealed that a major 1.5-kb PDXK transcript was expressed in all tissues tested.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QV LGFEVDAVNS VQFSNHTGYA HWKGQVLKSQ ELHELYEGLK VNDVNKYDYV LTGYTRDKSF LAMVVDIVRE LKQQNSRLVY VCDPVMGDKW NGEGSMYVPQ DLLPVYRDKV VPVADIITPN QFEAELLSGR KIHSQEEAFE VMDMLHCMGP DTVVITSSDL PSSQGSDYLI ALGSQRMRKP DGSTVTQRIR MEMRKVEAVF VGTGDLFAAM LLAWTHKHPD NLKVACEKTV SAMQHVLQRT IRCAKAEAGE GQKPS
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.