Podoplanin is a type-I integral membrane glycoprotein with diverse distribution in human tissues.
The physiological function of this protein may be related to its mucin-type character.
The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor.
The specific function of this protein has not been determined but it has been proposed as a marker of lung injury.
Alternatively spliced transcript variants encoding different isoforms have been identified.Highly expressed in placenta, lung, skeletal muscle and brain.
Weakly expressed in brain, kidney and liver.
In placenta, expressed on the apical plasma membrane of endothelium.
In lung, expressed in alveolar epithelium.
Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse PDPN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gly23-Val138 of mouse PDPN, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
