84-4432-03　PDIA2 (Protein Disulfide Isomerase A2, PDI-A2, PDA2, PDI, PDIR, PDIP, Protein Disulfide Isomerase,Pancreatic, Protein Disulfide Isomerase-Associated 2, Pancreas-specific protein disulfide isomerase) 200ul　364592

特徴

• Protein disulfide isomerase or PDI is an enzyme in the endoplasmic reticulum in eukaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold.
• Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions.
• Desilva et al.
• (1996) cloned PDIP from an insulinoma subtraction cDNA library.
• The deduced 511aa protein has a calculated molecular mass of about 56.6kD and contains 2 thioredoxin-like catalytic sites, a C-terminal ER retention sequence (KEEL), and 3 potential N-glycosylation sites.
• PDIP shares about 46% identity with bovine, mouse, rabbit, and human PDIs.
• Northern blot analysis detected a 2.0-kb transcript expressed exclusively in pancreas.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LF VVVDVAADNE HVLQYFGLKA EAAPTLRLVN LETTKKYAPV DGGPVTAASI TAFCHAVLNG QVKPYLLSQE IPPDWDQRPV KTLVGKNFEQ VAFDETKNVF VKFYAPWCTH CKEMAPAWEA LAEKYQDHED IIIAELDATA NELDAFAVHG FPTLKYFPAG PGRKVIEYKS TRDLETFSKF LDNGGVLPTE EPPEEPAAPF PEPPANSTMG SKEEL
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.