84-4432-01　［受注停止］PDHX (Pyruvate Dehydrogenase Complex Component X, E3BP, proX, PDX1, OPDX, DLDBP, E3-binding protein, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex) 200ul　364590

特徴

• The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex.
• For a detailed description of the pyruvate dehydrogenase complex.
• The mammalian PDH complex differs from that in E.
• coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53kD protein called protein X.
• Component X binds to the E3 component of the PDH complex
• The open reading frame encodes an 501aa protein sequence that is 20% identical with that encoded by the yeast PDX1 gene and 40% identical with that encoded by the lipoacyltransferase component E2 of the PDH (E2), and includes a lipoyl-bearing domain.
• Sequence comparisons showed that the PDHX protein contains an E3-binding domain.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- V QLKQTGKITE SRPTPAPTAT PTAPSPLQAT AGPSYPRPVI PPVSTPGQPN AVGTFTEIPA SNIRRVIAKR LTESKSTVPH AYATADCDLG AVLKVRQDLV KDDIKVSVND FIIKAAAVTL KQMPDVNVSW DGEGPKQLPF IDISVAVATD KGLLTPIIKD AAAKGIQEIA DSVKALSKKA RDGKLLPEEY QGGSFSISNL GMFGIDEFTA VINPPQACIL AVGRFRPVLK LTEDEEGNAK LQQRQLIT
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.